Journal:

Article Title: Gene-expression signature of benign monoclonal gammopathy evident in multiple myeloma is linked to good prognosis

doi: 10.1182/blood-2006-07-037077

Figure Lengend Snippet: Fifty-two SAM-defined genes are differentially expressed in NPC, MGUS, and MM

Article Snippet: 2.05 1.55 3.18 1.36 212038_s_at VDAC1 Ion channel for cytochrome c 2.62 1.56 3.17 1.42 208308_s_at GPI Energy metabolism 1.97 1.48 3.17 1.48 201013_s_at PA1CS DNA synthesis 2.81 1.43 3.17 1.45 202708_s_at HIST2H2BE Chromosome organization and biogenesis 2.35 1.71 3.14 2.11 215071_s_at HIST1H2AC Chromosome organization and biogenesis 3.47 3.62 3.13 1.97 225361_x_at LOC159090 Unknown 3.43 1.68 3.11 1.53 219366_at AVEN Antiapoptosis 2.63 1.76 3.10 1.47 209398_at H1ST1H1C Chromosome organization and biogenesis 5.39 5.53 3.09 2.02 221652_s_at C12orf11 Unknown; sarcoma antigen NY-SAR-95 2.07 1.37 3.06 1.57 225028_at LOC550643 Unknown 3.83 2.01 3.03 1.45 214214_s_at C1QBP Immunity 2.64 1.45 3.03 1.47 201577_at NME1 Nucleotide biosynthesis 3.57 1.62 2.99 1.58 218280_x_at H1ST2H2AA Chromosome organization and biogenesis 4.24 4.76 2.95 1.92 201479_at DKC1 Telomere maintenance 2.24 1.42 2.92 1.43 208864_s_at TXN Redox reactions 2.90 1.68 2.91 1.51 212297_at ATP13A3 Cation transport 2.64 1.42 2.88 1.54 222825_at OTUD6B Unknown 2.66 1.35 2.88 1.40 209267_s_at SLC39A8 Ion transport 3.41 1.86 2.88 1.56 217898_at C15orf24 Unknown 2.35 1.70 2.83 1.33 210275_s_at ZA20D2 Unknown 2.27 1.36 2.81 1.37 213485_s_at ABCC10 ATP-dependent efflux pump; multidrug resistance pump 3.17 1.51 2.76 1.34 200994_at IPO7 Nuclear trafficking 2.08 1.46 2.72 1.37 222428_s_at LARS Protein synthesis 3.64 1.68 2.71 1.32 202591_s_at SSBP1 Mitochondrial DNA replication 2.22 1.42 2.69 1.35 204244_s_at ASK Cell cycle 3.25 1.77 2.67 1.37 225916_at ZNF131 Gene transcription 2.47 1.39 2.63 1.36 202396_at TCERG1 Gene transcription 3.85 1.62 2.63 1.32 213340_s_at K1AA0495 Unknown −2.40 0.78 −2.52 0.75 206150_at TNFRSF7 Immune cell signaling −3.82 0.55 −2.56 0.75 228139_at RIPK3 Cell signaling −2.63 0.74 −2.59 0.77 220066_at CARD15 Antiapoptosis −4.02 0.50 −2.66 0.62 210347_s_at BCL11A Gene transcription 3.48 2.04 −2.67 0.64 232511_at RANBP2L1 Nuclear import 2.07 1.54 −2.71 0.64 214041_x_at RPL37A Ribosomal protein 2.22 1.80 −3.09 0.60 219371_s_at KLF2 Gene transcription 3.81 1.78 −3.11 0.66 202724_s_at FOXO1A Gene transcription 2.49 1.55 −3.27 0.64 215671_at PDE4B Drug metabolism 2.07 1.53 −3.30 0.59 219675_s_at UXS1 Glycosaminoglycan biosynthesis −2.33 0.76 2.97 1.35 Open in a separate window Genes are ordered based on the SAM score in the MM vs MGUS comparison.

Techniques: Binding Assay, DNA Synthesis

Journal: Scientific Reports

Article Title: p38α MAPK regulates proliferation and differentiation of osteoclast progenitors and bone remodeling in an aging-dependent manner

doi: 10.1038/srep45964

Figure Lengend Snippet: ( A ) TRAP staining showed that p38α deficiency promoted osteoclast differentiation in low cell density culture but slightly inhibited osteoclast differentiation in high cell density cultures. Monocytes were isolated from LysM-Cre; p38α f/f and control mice, plated at different densities, and cultured in the presence of RANKL and M-CSF. After 7 days, the cultures were stained for TRAP (left panels). Right panels: quantitation data. Scale bar, 200 μm. N = 3. ( B ) Quantitative PCR results showed that p38α deficiency promoted the expression of genes required for osteoclast differentiation at low cell densities. Monocytes described in Fig. 2A (low cell density) were collected and used to isolate total RNA, which was used to run quantitative PCR assays. N = 3. ( C ) Quantitative PCR results showed that p38α deficiency slightly inhibited the expression of genes required for osteoclast differentiation at high cell densities. ( D ) Western blot results showed that p38α deficient monocytes cultures at low densities exhibited an increase in activation of Tak1 and the protein levels of NF-κB isoforms p50/52 and p65. Scale bar, 100 μm. ( E ) Inhibition of Tak1 down-regulated NF-κB levels and suppressed osteoclast differentiation. Left panel: western blot showed that Tak1 inhibitor down-regulated NF-κB levels. Middle panel: Tak1 inhibitor suppressed osteoclast differentiation. Right panel: Quantitation data. N = 3. ( F ) Western blot results revealed that p38α deficient monocyte cultures at high densities showed a decrease in c-Fos protein levels. ( G ) p38α deficiency did not affect the resorbing activity of osteoclasts on dentine slices. WT and p38 deficient monocytes were induced to differentiate into osteoclasts by M-CSF and RANKL for 2 days and then counted and the same numbers of cells were plated onto dentine slices. After 7 days, the dentine slices were sonicated and stained with Gill’s hematoxylin. Right panel: quantitation data. Scale bar, 200 μm. N = 3. For all results in Fig. 2, P-values are based on Student’s t-test. *p < 0.05, **p < 0.01 when the value of mutant mice or cells was compared to that of control mice or cells, or the drugs-treated group compared to control group.

Article Snippet: The specific antibodies used in this study were: NF-κB p65 (Cell Signaling, 4767, Boston, MA), NF-κB p50/52 (Santa Cruz, sc-8414, California), p38α MAPK (Cell Signaling, 9212, Boston, MA), p38β (Cell Signaling, 2339, Boston, MA), p-p38MAPK (T180/182) (Cell Signaling, 9211, Boston, MA), p53 (c12) (Cell Signaling, 2524, Boston, MA), Tak1 (Cell Signaling, 4505, Boston, MA), p-Tak1(T184/187) (Cell Signaling, 4531, Boston, MA), Creb (Upstate, 05767, Boston, MA), p-Creb (Upstate, 6519, Boston, MA), ERK (Cell Signaling, 9107, Boston, MA), p-ERK (Cell Signaling, 9106s, Boston, MA), cFos (Calbiochem, pc38, Darmstadt, Germany), p-cFos (Santa Cruz, sc-81485, California), and β-ACTIN (Santa Cruz, sc-81178, California).

Techniques: Staining, Isolation, Control, Cell Culture, Quantitation Assay, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Activation Assay, Inhibition, Activity Assay, Sonication, Mutagenesis